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41.
W. A. Roth Fr Müller A. Eucken L. Meyer W. Wien F. Harm H. Lenz W. P. White W. Swietoslawski A. Dorabialska S. G. Lipsett F. M. G. Johnson O. Maass H. C. Dickinson E. F. Mueller A. Goetz H. v. Wartenberg H. Moehl F. Henning E. Berl H. Schildwächter K. Endell L. C. Glaser K. B. Eisenberg W. Eitel B. Lange M. Berek A. Szegvary R. Anschütz A. Kullmann H. Rheinboldt M. Kircheisen K. Rast O. Dischendorfer F. Emich C. M. Cooper E. V. Fasce A. W. Laubengayer R. B. Corey M. Wagenaar A. Mayrhofer K. Herzig K. Zauder P. Manicke H. Lauth 《Analytical and bioanalytical chemistry》1929,76(5-6):216-231
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Rieux L Lubda D Niederländer HA Verpoorte E Bischoff R 《Journal of chromatography. A》2006,1120(1-2):165-172
Proteomic studies have stimulated the development of novel stationary phases in miniaturised chromatographic columns that permit high linear flow velocities and exhibit high resolving power. In this work, a 50-microm reversed-phase silica-based monolith was chromatographically characterised for its use in proteomics applications using a nanoLC-MS set-up. It showed high efficiency for the separation of tryptic peptides under isocratic elution conditions (HETP(min)=5-10 microm at 2.4 mm/s). Flow rates up to 1.95 microL/min (18.4 mm/s) and gradient slopes up to an unusually fast 9% could be used. This resulted in rapid separations of peptide mixtures, with peak widths at half height of between 5 and 10 s. The 50-microm monolithic column was used to analyse depleted serum from a cervical cancer patient at a throughput of one sample per 30 min. 相似文献
44.
van Duijn E Simmons DA van den Heuvel RH Bakkes PJ van Heerikhuizen H Heeren RM Robinson CV van der Vies SM Heck AJ 《Journal of the American Chemical Society》2006,128(14):4694-4702
It has been suggested that the bacterial GroEL chaperonin accommodates only one substrate at any given time, due to conformational changes to both the cis and trans ring that are induced upon substrate binding. Using electrospray ionization mass spectrometry, we show that indeed GroEL binds only one molecule of the model substrate Rubisco. In contrast, the capsid protein of bacteriophage T4, a natural GroEL substrate, can occupy both rings simultaneously. As these substrates are of similar size, the data indicate that each substrate induces distinct conformational changes in the GroEL chaperonin. The distinctive binding behavior of Rubisco and the capsid protein was further investigated using tandem mass spectrometry on the intact 800-914 kDa GroEL-substrate complexes. Our data suggest that even in the gas phase the substrates remain bound inside the GroEL cavity. The analysis revealed further that binding of Rubisco to the GroEL oligomer stabilizes the chaperonin complex significantly, whereas binding of one capsid protein did not have the same effect. However, addition of a second capsid protein molecule to GroEL resulted in a similar stabilizing effect to that obtained after the binding of a single Rubisco. On the basis of the stoichiometry of the GroEL chaperonin-substrate complex and the dissociation behavior of the two different substrates, we hypothesize that the binding of a single capsid polypeptide does not induce significant conformational changes in the GroEL trans ring, and hence the unoccupied GroEL ring remains accessible for a second capsid molecule. 相似文献
45.
This obituary for Israel Gohberg consists of a general introduction, separate contributions of the six authors, all of whom worked closely with him, and a final note. The material gives an impression of the life of this great mathematician, of his monumental impact in the areas he worked in, of how he cooperated with colleagues, and of his ability to stimulate people in their mathematical activities. 相似文献
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Abstract— Unirradiated phage T4v1 may fail to produce viable progeny in cells which are co-infected with u.v.-irradiated homologous particles. The extent of this effect, called suppression , is positively correlated with the multiplicity of infection of the irradiated phage and with the U.V. dose. The suppression reaches a maximum level at about 30–600 lethal hits. Quantitative evaluation of the results shows that in some complexes one irradiated phage particle is sufficient to suppress an unirradiated phage. Two hypotheses are discussed to explain the results. (a) Lethal u.v.-damages are incorporated into the unirradiated phage genome by genetic recombination; ( b ) Genetic subunits (e.g. cistrons or operons) of the u.v.-irradiated phages produce informationally incorrect messenger RNA molecules, which compete with the correct ones from the unirradiated phage in the protein-synthetizing system. Hypothesis (6) appears to be more adequate to explain the experimental results. 相似文献
48.
Transcyclometalation has been successfully applied for the multi-platination and -ruthenation of 'chartwheel'-type ligand systems containing six potential metal binding sites, thus providing a method for multiple metal-carbon bond formation via C-H bond activation which is superior to established cyclometalation protocols. 相似文献
49.
SUBSTRATE SPECIFICITY OF A BACTERIAL UV ENDONUCLEASE AND THE OVERLAP WITH IN VITRO PHOTOENZYMATIC REPAIR 总被引:2,自引:0,他引:2
Abstract— The action of an endonuclease from Micrococcus luteus , that operates on ultraviolet (UV) radiation damage, overlaps greatly with that of the yeast photoreactivating enzyme: homo and hetero cyclobutyl pyrimidine dimers in DNA are substrate for both enzymes, but pyrimidine adducts or the 'spore photoproduct' in DNA are not.
As expected from this overlap, the action of the two enzymes is mutually interfering: single-strand nicks introduced by the endonuclease effectively preclude photoreactivation; conversely, formation of a photoreactivating enzyme-dimer complex can prevent nicking by the UV endonuclease. While complex formation between photoreactivating enzyme and dimers in UV-endonuclease-treated DNA is apparently normal, the light-dependent repair step either fails to occur or proceeds at a very low rate. Hence, besides the requirement of a minimum chain length for the function of the photoreactivating enzyme, there is the additional restriction on the position of the dimer in a polynucleotide strand.
Finally, rough approximations of the rate constants, k1 and k 2 , for the UV endonuclease indicate that the in vitro UV-endonuclease-dimer complex is relatively unstable, with dissociation of the complex being more probable than hydrolysis of the phosphodiester bond. 相似文献
As expected from this overlap, the action of the two enzymes is mutually interfering: single-strand nicks introduced by the endonuclease effectively preclude photoreactivation; conversely, formation of a photoreactivating enzyme-dimer complex can prevent nicking by the UV endonuclease. While complex formation between photoreactivating enzyme and dimers in UV-endonuclease-treated DNA is apparently normal, the light-dependent repair step either fails to occur or proceeds at a very low rate. Hence, besides the requirement of a minimum chain length for the function of the photoreactivating enzyme, there is the additional restriction on the position of the dimer in a polynucleotide strand.
Finally, rough approximations of the rate constants, k
50.
Lensen MC van Dingenen SJ Elemans JA Dijkstra HP van Klink GP van Koten G Gerritsen JW Speller S Nolte RJ Rowan AE 《Chemical communications (Cambridge, England)》2004,(7):762-763
A giant porphyrin disc (M(w)= 15 kDa) has been synthesized and its self-assembly behaviour at an interface studied by liquid STM which reveals the presence of huge domains (>400 x 400 nm2) of very well ordered and molecularly resolved columnar stacks. 相似文献